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Updated: May 31, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Quantification of hepatitis B virus HBsAg: clinical implications]
Maria Buti1, Francisco Rodríguez Frías, Rafael Esteban
1Servicio de Hepatología, Hospital Universitario Vall d'Hebron, Barcelona, Spain. Mbuti@vhebron.net
Abstract:
The surface antigen of hepatitis B virus (HBsAg) is the main serological marker of HBV infection since its discovery almost 50 years ago. Currently the quantification of HBsAg has acquired special relevance as there are commercial tests to measure its levels. Several studies have shown that in patients treated with pegylated interferon alfa the fall of HBsAg levels predicts the loss of HBsAg and persistent virologic response. The role of the quantification of HBsAg in the treatment with nucleoside analogues is still not well understood and requires further studies.
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