Related Experiment Video
Updated: May 31, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
Published on: June 13, 2014
Physical and functional cooperation of neural cell adhesion molecule and β1-integrin in neurite outgrowth induction
Athanasios K Petridis1, Sotiris N Nikolopoulos, Abderrahman El-Maarouf
1Memorial Sloan Kettering Cancer Center, New York, USA. opticdisc@aol.com
Abstract:
Neural cell adhesion molecule (NCAM) and β1-integrin are both involved in cell differentiation, with changes in the expression of these two molecules correlating with changes in the malignancy of tumor cells. There is a known functional correlation between NCAM and β1-integrin in adhesion and also neurite outgrowth in tumor cells. In the present study, we used immunostaining and immunoprecipitation studies to demonstrate that isoform 120 of NCAM associates physically as well as functionally with β1-integrin in the induction of neurite outgrowth in SH-SY5Y-human neuroblastoma cells. The interaction between these two molecules is mandatory for neurite outgrowth. NCAM blockage completely inhibits the effects of β1-integrin on neurite outgrowth. These findings further our understanding of the interactions between NCAMs and integrins in malignancy.
Related Concept Videos
Intracellular Signaling Affects Focal Adhesions
Some...
Activation of Integrins
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Integrins
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Cell Adhesion Molecules - Types and Functions
CAM Families
The Integrin family of proteins is primarily involved in a...
Cell Adhesion Molecules - Types and Functions
CAM Families
The Integrin family of proteins is primarily involved in a...

