Nuclear formation in a Drosophila cell-free system
Related Experiment Video
Updated: Oct 2, 2026
12:48
Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
Published on: March 25, 2014
1Department of Pharmacological Sciences, School of Medicine, State University of New York, Stony Brook 11794-8651.
Experimental Cell Research
|November 1, 1990
Abstract:
A cell-free preparation obtained from 0- to 5-h-old Drosophila melanogaster embryos induces chromatin decondensation and nuclear formation from demembranated Xenopus sperm. Newly formed nuclei have a peripheral lamina, a double membrane, and structures resembling pore complexes. Indirect immunofluorescence analyses demonstrate the association of Drosophila lamins and DNA topoisomerase II with newly assembled nuclei.
