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Updated: Jan 25, 2026

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
Published on: June 30, 2023
Compact Dry(R) X-BC for the enumeration of Bacillus cereus in food samples
Hajime Teramura1, Masafumi Uchida, Hidemasa Kodaka
1Nissui Pharmaceutical Co., Ltd., Yuki, Ibaraki, Japan. h-teramura@yki.nissui-pharm.jp
Abstract:
We evaluated the effectiveness of using Compact Dry(R) X-BC (CD-XBC), a ready-to-use and self-diffusing dry medium sheet culture system based on a novel detection principle, for the detection and enumeration of Bacillus cereus. All 13 B. cereus strains, which were studied for the inclusivity study, grew as blue/green colonies on the CD-XBC. When 3 yeast strains and 103 bacterial strains other than B. cereus were tested for the exclusivity study, 5 strains formed white colonies, and 4 strains formed blue/green colonies, while 94 other strains failed to grow. The 4 strains that formed blue/green colonies were B. thuringiensis, which is known to have the same biochemical features as B. cereus. The CD-XBC method was compared with the MYP agar method (MYP) and the NGKG agar method (NGKG) in 130 artificially contaminated food samples. The correlation coefficients between CD-XBC and MYP, and CD-XBC and NGKG were 0.972 and 0.971, respectively.
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