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Related Concept Videos

Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Related Experiment Video

Updated: May 31, 2026

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

Stem cell culture engineering - process scale up and beyond.

Shikha Sharma1, Ravali Raju, Siguang Sui

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA.

Biotechnology Journal
|July 2, 2011
PubMed
Summary

Stem cell production for regenerative medicine needs robust, scalable processes. Controlling traditional and non-traditional variables ensures consistent cell product quality for clinical applications.

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Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Cell Therapy Manufacturing

Background:

  • Stem cell research advances clinical regenerative medicine prospects.
  • Clinical stem cell use necessitates transitioning lab practices to robust manufacturing.

Purpose of the Study:

  • To outline requirements for robust stem cell bioprocessing.
  • To identify strategies for ensuring consistent cell product quality.

Main Methods:

  • Reviewing stem cell handling from isolation to formulation.
  • Drawing parallels with established biomanufacturing, e.g., recombinant proteins, vaccines.
  • Identifying key process variables for control.

Main Results:

  • Stem cell manufacturing requires extensive ex vivo handling and scalable operations.
  • Cell source variability and product quality fluctuations are inherent challenges.
  • Process robustness relies on controlling traditional (chemical, physical) and non-traditional (signaling pathway modulators) variables.

Conclusions:

  • Robust stem cell bioprocessing is critical for clinical translation.
  • Consistent cell product quality depends on controlling both conventional and novel process parameters.
  • Lessons from biologics manufacturing can inform stem cell process development.