Related Experiment Video
Updated: May 31, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
Published on: April 9, 2014
Spatial filter based 3D resolution improvement and polarization properties of multiphoton
Shilpa Dilipkumar1, Alberto Diaspro, Partha Pratim Mondal
1Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore 560012, India.
Abstract:
Three-dimensional (3D) resolution improvement in multi-photon multiple-excitation-spot-optical microscopy is proposed. Specially designed spatial filter is employed for improving the overall 3D resolution of the imaging system. An improvement up to a factor of 14.5 and sub-femto liter volume excitation is achieved. The system shows substantial sidelobe reduction (<4%) due to the non-linear intensity dependence of multiphoton process. Polarization effect on x-oriented and freely rotating dipoles shows dramatic change in the field distribution at the focal-plane. The resulting point-spread function has the ability to produce several strongly localized polarization dependent field patterns which may find applications in optical engineering and bioimaging.
More Related Videos
Related Concept Videos
Super-resolution Fluorescence Microscopy
Confocal Fluorescence Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

