Related Experiment Video
Updated: May 31, 2026
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
Rapid genotyping assays for the identification and differentiation of Yersinia ruckeri biotype 2 strains
1National Center for Cool and Cold Water Aquaculture, Agricultural Research Service, US Department of Agriculture, Kearneysville, WV 25430, USA. tim.welch@ars.usda.gov
Aims:
To establish PCR-based assays for the rapid identification and differentiation of each of four known biotype 2 (BT2) phenotype-causing alleles in Yersinia ruckeri strains currently circulating in Europe and the United States.
Methods And Results:
Novel assays were developed relying on detection of mutant allele-specific changes in restriction enzyme cleavage sites within targeted PCR products. The developed assays were validated against isolates previously genotyped by DNA sequencing.
Conclusions:
The described methods were specific, rapid and simple to perform and interpret.
Significance And Impact Of The Study:
The developed genotyping assays provide a valuable tool for identification and differentiation of specific BT2 strains of Y. ruckeri. These assays will be critical for the design and validation of new vaccines or other measures meant to control BT2 strains.
