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Analysis with restriction endonucleases recognizing 4- or 5-base-pair sequences of human adenovirus type 3 isolated
1Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Abstract:
A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3 subgenome types, respectively. During this observation period, two epidemics of Ad3 infection relating to epidemic keratoconjunctivitis occurred. The prevalent Ad3g strains isolated during the epidemic in 1983 belonged to a single subgenome type. However, in the course of the Ad3f epidemic in 1986, alteration of one subgenome type of the prevalent strain to a different subgenome type which was presumed to be a derivative of the former was observed. In the years in which only a few Ad3 strains were isolated, such Ad3f strains were classified into different subgenome types. However, all Ad3g strains except two belonged to the same subgenome type.
Insights
Epidemic keratoconjunctivitis outbreaks were studied in Sapporo, Japan. Adenovirus type 3 (Ad3) strains showed distinct genomic profiles, with one type altering during an epidemic.
Area of Science:
- Ophthalmology
- Virology
- Molecular Biology
Background:
- Epidemic keratoconjunctivitis is a contagious eye infection often caused by adenoviruses.
- Adenovirus type 3 (Ad3) is a common cause of epidemic keratoconjunctivitis.
- Genomic diversity of Ad3 strains can influence disease outbreaks.
Purpose of the Study:
- To characterize the genomic types of adenovirus type 3 (Ad3) strains.
- To investigate the evolution of Ad3 strains during epidemic keratoconjunctivitis outbreaks.
- To understand the relationship between Ad3 genomic types and disease epidemiology.
Main Methods:
- Restriction endonuclease analysis (Hinf1 and TaqI) was used to classify Ad3 strains.
- Genomic typing of 49 Ad3 strains isolated from patients between 1983 and 1986.
- Epidemiological data from two Ad3 infection outbreaks were analyzed.
Main Results:
- Forty-nine Ad3 strains were classified into genome types Ad3f (26 strains) and Ad3g (23 strains).
- Eleven and 3 subgenome types were identified using Hinf1 and TaqI, respectively.
- A shift in subgenome type was observed within the Ad3f strains during the 1986 epidemic, suggesting strain evolution.
Conclusions:
- Ad3 strains exhibit genomic diversity, with distinct prevalent types during different epidemic periods.
- The observed alteration in Ad3f subgenome type during an epidemic highlights viral adaptability.
- Understanding Ad3 genomic variations is crucial for tracking and managing epidemic keratoconjunctivitis.