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Updated: May 31, 2026

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Published on: July 19, 2022
Influence of lipid membrane rigidity on properties of supporting polymer
Michael S Jablin1, Manish Dubey, Mikhail Zhernenkov
1Lujan Neutron Scattering Center, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
Abstract:
Temperature-sensitive hydrogel polymers are utilized as responsive layers in various applications. Although the polymer's native characteristics have been studied extensively, details concerning its properties during interaction with biorelated structures are lacking. This work investigates the interaction between a thermoresponsive polymer cushion and different lipid membrane capping layers probed by neutron reflectometry. N-isopropylacrylamide copolymerized with methacroylbenzophenone first supported a lipid bilayer composed of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and subsequently 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The polymer-membrane systems were investigated above and below the polymer transition temperature (37 and 25°C). Although the same cushion supported each lipid membrane, the polymer hydration profile and thickness were markedly different for DPPE and DPPC systems. Because DPPE and DPPC have different bending rigidities, these results establish that the polymer-membrane interaction is critically mediated by the mechanics of the membrane, providing better insight into cell-hydrogel interactions.
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