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Effect of soil clay content on RNA isolation and on detection and quantification of bacterial gene transcripts in

A Novinscak1, M Filion

  • 1Université de Moncton, Biology Department, Moncton, New Brunswick, Canada.

Applied and Environmental Microbiology
|July 5, 2011
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...