Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Fungi

Overview of Fungi

Fungi are a diverse group of eukaryotes more closely related to animals than other eukaryotes. Fungal cell walls comprise chitin, a polysaccharide that provides structural strength, and glucans, which contribute to flexibility and integrity. Other polysaccharides, such as mannans and galactosans, may supplement or replace chitin in some fungi. These adaptations, along with their preference for acidic environments and tolerance for high osmotic pressure, enable fungi to thrive in various...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Microbe-Plant Interactions

Microbe-Plant Interactions

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Estimating the percentage and number of people with indications for HIV pre-exposure prophylaxis (PrEP) within two key populations, Canada, 2021.

Canadian journal of public health = Revue canadienne de sante publique·2026

Same author

Estimating the population size of people who inject drugs in Canada, 2021.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2025

Same author

Stabilisation of Tomato Yellow Leaf Curl China Virus Infectious Clones Through Micro-Homology Mediated End Joining.

Plant biotechnology journal·2025

Same author

Prevalence and awareness of hepatitis B and hepatitis C and vaccine-induced immunity to hepatitis B: Findings from the Canadian Health Measure Survey, 2016-2019.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2025

Same author

Estimated prevalence of hepatitis B and C among immigrants in Canada.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2025

Same author

National hepatitis B and C estimates for 2021: Measuring Canada's progress towards eliminating viral hepatitis as a public health concern.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2025

Search research articles

Related Experiment Video

Updated: May 31, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

RNA interference in fungi: pathways, functions, and applications.

Yunkun Dang¹, Qiuying Yang, Zhihong Xue

¹Department of Physiology, ND13.214A, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9040, USA. Yi.Liu@UTsouthwestern.edu

Eukaryotic Cell

|July 5, 2011

Summary

Fungal RNA interference (RNAi) pathways are crucial for gene regulation and defense. Diverse small RNA biogenesis in fungi offers insights into eukaryotic systems and potential research tools.

More Related Videos

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Published on: March 9, 2022

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Published on: April 26, 2024

Related Experiment Videos

Last Updated: May 31, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Published on: March 9, 2022

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Published on: April 26, 2024

Area of Science:

Molecular Biology
Genetics
Mycology

Background:

Small RNA molecules (20-30 nucleotides) mediate gene regulation and genomic defense through RNA interference (RNAi) pathways.
The core RNAi machinery includes Dicer, Argonaute, and RNA-dependent RNA polymerase.
In fungi, RNAi pathways are involved in genomic defense, heterochromatin formation, and gene regulation.

Purpose of the Study:

To review the current understanding of fungal RNAi-related pathways and their functions.
To focus on the role of RNAi in filamentous fungi.
To explore the potential of RNAi as a tool in fungal research.

Main Methods:

Review of existing literature on fungal RNAi pathways.
Analysis of studies on model fungi like Schizosaccharomyces pombe and Neurospora.
Focus on small RNA biogenesis and its implications.

Main Results:

Fungal RNAi pathways exhibit significant diversity in small RNA biogenesis.
These pathways are essential for adaptation to various environmental conditions.
Studies provide insights into general eukaryotic RNAi mechanisms.

Conclusions:

Fungal RNAi pathways are versatile and play critical roles in cellular processes.
Understanding fungal RNAi enhances knowledge of eukaryotic gene regulation and defense.
RNAi presents a valuable tool for advancing fungal research.