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Updated: May 31, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Lysinibacillus macroides sp. nov., nom. rev.

An Coorevits^1,2, Anna E Dinsdale³, Jeroen Heyrman¹

¹Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.

International Journal of Systematic and Evolutionary Microbiology

|July 5, 2011

Summary

This study reclassifies 'Bacillus macroides' as Lysinibacillus macroides sp. nov. based on genomic and phenotypic data, restoring its standing in bacterial nomenclature.

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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

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Area of Science:

Microbiology
Bacterial Taxonomy
Genomics

Background:

'Bacillus macroides' ATCC 12905(T) lost standing in bacterial nomenclature due to its exclusion from the Approved Lists of Bacterial Names.
The strain was originally isolated from cow dung in 1947.

Purpose of the Study:

To re-evaluate the taxonomic status of 'Bacillus macroides' ATCC 12905(T).
To determine the genomic and phenotypic characteristics of the strain for accurate classification.

Main Methods:

DNA-DNA relatedness experiments were performed.
16S rRNA gene sequence analysis was conducted.
Peptidoglycan type analysis and phenotypic characterization were carried out.

Main Results:

'Bacillus macroides' is genomically distinct from related Lysinibacillus species, with 16S rRNA gene sequence similarities ranging from 98.5% to 99.2%.
The strain possesses the A4α L-Lys-D-Asp peptidoglycan type, characteristic of the Lysinibacillus genus.
Phenotypic data further supported the reclassification.

Conclusions:

The strain LMG 18474(T) (= DSM 54(T) = ATCC 12905(T)) is validly described as Lysinibacillus macroides sp. nov., nom. rev.
This reclassification restores the standing of the bacterium in formal bacteriological nomenclature.