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Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software
Published on: July 24, 2021
Differential accumulation of nif structural gene mRNA in Azotobacter vinelandii
Trinity L Hamilton1, Marty Jacobson, Marcus Ludwig
1Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA.
Journal of Bacteriology
|July 5, 2011
Abstract:
Northern analysis was employed to investigate mRNA produced by mutant strains of Azotobacter vinelandii with defined deletions in the nif structural genes and in the intergenic noncoding regions. The results indicate that intergenic RNA secondary structures effect the differential accumulation of transcripts, supporting the high Fe protein-to-MoFe protein ratio required for optimal diazotrophic growth.
