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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: May 31, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

MicroRNA function in myeloid biology.

Ryan M O'Connell1, Jimmy L Zhao, Dinesh S Rao

  • 1California Institute of Technology, Pasadena, CA, USA.

Blood
|July 5, 2011
PubMed
Summary

MicroRNAs (miRNAs) are crucial regulators of myeloid development and activation. Dysregulation of specific miRNAs, including miR-125, miR-146, miR-155, and miR-223, contributes to myeloid diseases.

Area of Science:

  • Molecular Biology
  • Hematology
  • Genetics

Background:

  • MicroRNAs (miRNAs) have emerged as critical regulators in biological processes.
  • Their roles in hematopoiesis, cancer, and various diseases are increasingly understood.
  • Myeloid development presents unique features revealed through miRNA manipulation.

Purpose of the Study:

  • To review the specific roles of four key miRNAs (miR-125, miR-146, miR-155, miR-223) in myeloid development and activation.
  • To correlate the functions of these miRNAs with their dysregulation in myeloid-related diseases.
  • To detail their targets and regulatory networks within the myeloid lineage.

Main Methods:

  • Literature review focusing on experimental studies of miRNA function in myeloid development.

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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Related Experiment Videos

Last Updated: May 31, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

  • Analysis of data linking miRNA loss-of-function or overexpression to myeloid pathologies.
  • Examination of miRNA targets and their integration into regulatory networks.

    • Main Results:

    • All four reviewed miRNAs (miR-125, miR-146, miR-155, miR-223) significantly impact myelopoiesis.
    • Altered expression (loss-of-function or overexpression) of these miRNAs results in pathological phenotypes in the myeloid lineage.
    • These miRNAs exert their functions at distinct developmental stages and are part of complex regulatory networks.

    Conclusions:

    • Specific miRNAs play vital roles in normal myeloid development and activation.
    • Dysregulation of miR-125, miR-146, miR-155, and miR-223 is associated with myeloid diseases.
    • Understanding these miRNAs' functions and networks is crucial for therapeutic strategies in myeloid disorders.