Related Experiment Video
Updated: May 31, 2026

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis
Published on: October 14, 2025
CIN85 interacting proteins in B cells-specific role for SHIP-1
Tom Büchse1, Nikolaus Horras, Eva Lenfert
1Institute of Medical Biochemistry and Molecular Biology, Medical Faculty, University of Rostock, Schillingallee 70, 18057 Rostock, Germany. tom.buechse@med.uni-rostock.de
Cbl-interacting protein 85 (CIN85) regulates receptor tyrosine kinase signaling in B lymphocytes. CIN85 interacts with SHIP-1, potentially enhancing the down-regulation of phosphatidylinositol-3,4,5-trisphosphate levels.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Cbl-interacting protein 85 (CIN85) is a key adaptor protein regulating receptor tyrosine kinase (RTK) signaling.
- CIN85 enhances RTK-activated endocytosis and reduces phosphatidylinositol-3-kinase (PI3K)-induced phosphatidylinositol-3,4,5-trisphosphate (PIP3) production.
- CIN85 expression is reported in primary splenic B lymphocytes and B-lymphoma cell lines.
Purpose of the Study:
- To investigate the role and interactions of CIN85 in B lymphocytes.
- To identify novel CIN85-interacting proteins in B cells.
- To elucidate the functional significance of CIN85-SHIP-1 interaction in B cell signaling.
Main Methods:
- Western blotting and co-immunoprecipitation to study protein associations.
- Pull-down proteomics to identify CIN85 interactors.
- Analysis of CIN85 and SHIP-1 interaction domains.
Main Results:
- Cross-linking of the B cell antigen receptor increased CIN85 association with Cbl.
- Proteomics identified 51 CIN85-interacting proteins in B cells, including novel partners.
- SH2-containing inositol phosphatase 1 (SHIP-1) constitutively co-precipitated with CIN85 via its SH3 domains and a specific C-terminal region.
Conclusions:
- CIN85 is expressed in B lymphocytes and interacts with Cbl upon B cell receptor stimulation.
- A novel interaction between CIN85 and SHIP-1 was identified in B cells.
- The CIN85-SHIP-1 interaction may synergistically down-regulate PI3K/PIP3 signaling in lymphocytes.
More Related Videos
11:06Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
Published on: September 20, 2017
09:40Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Published on: September 28, 2018
Related Concept Videos
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Assembly of Signaling Complexes
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
Coat Assembly and GTPases
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Diversity of Antigen Receptors
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...