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Updated: May 31, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
Selective removal of promoter nucleosomes by the RSC chromatin-remodeling complex
Yahli Lorch1, Joachim Griesenbeck, Hinrich Boeger
1Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA. lorch@stanford.edu
Abstract:
Purified chromatin rings, excised from the PHO5 locus of Saccharomyces cerevisiae in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading frame (ORF) were retained. Treatment of the repressed rings with histone deacetylase diminished the removal of promoter nucleosomes. These findings point to a principle of promoter chromatin remodeling for transcription, namely that promoter specificity resides primarily in the nucleosomes rather than in the remodeling complex that acts upon them.
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