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Rhodamine B aggregation in self-assembled multilayers induced by polyelectrolyte and interfacial fluorescence
Sun Xiangying1, Liu Bin, Zhang Ying
1College of Material Science and Engineering, Huaqiao University, Xiamen 361021, China. sunxy@hqu.edu.cn
Talanta
|July 6, 2011
Summary
Rhodamine B aggregation on modified quartz surfaces alters its fluorescence. This enables highly sensitive, label-free DNA detection using PSS-modified self-assembled monolayers.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biophysics
Background:
- Rhodamine B is a fluorescent dye with applications in sensing.
- Self-assembled monolayers (SAMs) offer platforms for interfacial studies.
- Polyanions like sodium poly(4-styrenesulfonate) (PSS) can influence dye aggregation and photophysics.
Purpose of the Study:
- To investigate the photophysical properties of Rhodamine B.
- To explore the effect of PSS on Rhodamine B aggregation and fluorescence.
- To develop a sensitive method for label-free DNA detection.
Main Methods:
- Fabrication of Quartz/APES/PSS/RB SAMs.
- Spectroscopic analysis of Rhodamine B photophysics.
- Interfacial fluorescence sensing of DNA.
Main Results:
- Rhodamine B exhibited aggregation in Quartz/APES/PSS/RB SAMs.
- Fluorescence behavior of aggregated Rhodamine B differed from non-aggregated states.
- The developed SAMs demonstrated extremely high sensitivity for label-free DNA sensing.
Conclusions:
- PSS induces aggregation of Rhodamine B on SAMs, altering its fluorescence.
- The constructed SAMs provide a sensitive platform for label-free interfacial DNA detection.
