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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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Related Experiment Video

Updated: May 31, 2026

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

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Immuno-imaging using nanobodies.

Ilse Vaneycken1, Matthias D'huyvetter, Sophie Hernot

  • 1In vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.

Current Opinion in Biotechnology
|July 6, 2011
PubMed
Summary

Radiolabeled nanobodies are emerging as powerful tools for immuno-imaging, offering fast and specific disease targeting. Their unique properties enable patient-friendly imaging protocols with short-lived radioisotopes.

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Last Updated: May 31, 2026

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Area of Science:

  • Medical imaging
  • Immunology
  • Nanotechnology

Background:

  • Immuno-imaging combines imaging techniques with targeted probes for disease study.
  • Nanobodies, small antibody fragments, offer rapid and specific in vivo targeting.

Purpose of the Study:

  • To review the current status of radiolabeled nanobodies as targeting probes for immuno-imaging.
  • To highlight the potential of nanobodies as next-generation imaging agents.

Main Methods:

  • Review of preclinical studies and current research on radiolabeled nanobodies.
  • Focus on nanobody properties relevant to immuno-imaging applications.

Main Results:

  • Nanobodies demonstrate fast and specific in vivo targeting.
  • Imaging is feasible as early as 1 hour post-injection.
  • Enables the use of short-lived radioisotopes for improved safety and patient protocols.

Conclusions:

  • Radiolabeled nanobodies show significant promise for immuno-imaging.
  • Their properties support the development of patient-friendly and safe imaging protocols.
  • Nanobodies represent a potential advancement in diagnostic imaging probes.