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Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
Published on: August 2, 2011
Attempts to detect West Nile virus in wild birds in Poland
Jowita Samanta Niczyporuk1, Elżbieta Samorek-Salamonowicz, Wojciech Kozdruń
1National Veterinary Research Institute Puławy, Department of Poultry Viral Diseases, Pulawy, Poland. jowita.niczyporuk@piwet.pulawy.pl
Abstract:
The aim of the study was to attempt the detection of West Nile virus (WNV) in wild birds in Poland. Forty-eight species of 1912 wild birds were used for the investigations. The birds were derived from various locations in Poland from early spring till late autumn of the years 2009-2011. The brain samples were homogenised and cellular RNA was isolated. Two methods (RT-PCR and nested RT-PCR) were used. The presence of WNV RNA was not detected in the samples examined. Additionally, a short analysis of the epizootiological situation regarding the presence of WNV in Poland is presented.

