Related Experiment Video
Updated: May 31, 2026

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
Published on: July 27, 2016
Covalent heparin modification of a polysulfone flat sheet membrane for selective removal of low-density lipoproteins:
Jing Li1, Xiao-Jun Huang, Jing Ji
1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Abstract:
A simple, convenient and economical method for the heparinization of PSf membranes is described, with the aim of preparing an LDL adsorber for simultaneous LDL apheresis and hemodialysis. An atmospheric pressure glow discharge generator is used to activate the PSf membrane surface, with subsequent chemical binding of heparin in the presence of EDC and NHS. ATR-FTIR spectroscopy and XPS measurements confirm successful surface modification. The PSf-Hep membrane shows good blood compatibility, with a relatively low amount and normal morphology of adherent platelets. ELISA results indicate that the PSf-Hep membrane exhibits excellent selective affinity for LDL in single and binary protein solutions, suggesting potential applications in hemodialysis with simultaneous LDL removal.
Related Concept Videos
Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration
Anticoagulant Drugs: Low-Molecular-Weight Heparins

