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Updated: May 31, 2026
CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Electrochemical and spectroscopic study on the interaction between isoprenaline and DNA using multivariate curve
Yongnian Ni1, Min Wei, Serge Kokot
1The State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China. ynni@ncu.edu.cn
Abstract:
Interaction of isoprenaline (ISO) with calf-thymus DNA was studied by spectroscopic and electrochemical methods. The behavior of ISO was investigated at a glassy carbon electrode (GCE) by cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV); ISO was oxidized and an irreversible oxidation peak was observed. The binding constant K and the stoichiometric coefficient m of ISO with DNA were evaluated. Also, with the addition of DNA, hyperchromicity of the UV-vis absorption spectra of ISO was noted, while the fluorescence intensity decreased significantly. Multivariate curve resolution-alternating least squares (MCR-ALS) chemometrics method was applied to resolve the combined spectroscopic data matrix, which was obtained by the UV-vis and fluorescence methods. Pure spectra of ISO, DNA and ISO-DNA complex, and their concentration profiles were then successfully obtained. The results indicated that the ISO molecule intercalated into the base-pairs of DNA, and the complex of ISO-DNA was formed.
