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Inducing Apical Periodontitis in Mice
Published on: August 6, 2019
Local inflammatory markers and systemic endotoxin in aggressive periodontitis
L M Shaddox1, J Wiedey, N L Calderon
1Department of Periodontology, University of Florida College of Dentistry, PO Box 100434, Gainesville, FL 32610-0434, USA. lshaddox@dental.ufl.edu
Journal of Dental Research
|July 7, 2011
Summary
Systemic endotoxin levels are elevated in localized aggressive periodontitis (LAP), correlating with increased inflammatory mediators in gingival crevicular fluid and disease severity. This links systemic factors to local inflammation in LAP.
Area of Science:
- Periodontology
- Immunology
- Oral Microbiology
Background:
- Localized aggressive periodontitis (LAP) involves complex local and systemic factors.
- Previous research indicated a systemic hyper-inflammatory response to bacterial endotoxin in LAP.
- Mechanisms linking systemic factors to local periodontal inflammation remain unclear.
Purpose of the Study:
- To identify specific cyto/chemokines in gingival crevicular fluid (GCF) associated with LAP.
- To quantify systemic endotoxin levels in LAP patients.
- To evaluate correlations between systemic endotoxin, local inflammatory mediators, and clinical parameters in LAP.
Main Methods:
- Collected clinical data, GCF, and peripheral blood from 34 LAP patients, 10 healthy siblings, and 9 healthy unrelated controls.
- Quantified nine cyto/chemokines in GCF and systemic endotoxin levels in plasma.
- Performed correlation analysis between clinical parameters, GCF mediators, and plasma endotoxin.
Main Results:
- Nine mediators (e.g., TNFα, IL-1β, IL-6) were significantly elevated in GCF from LAP sites compared to healthy sites.
- Systemic endotoxin levels were 4-5 times higher in LAP patients than in healthy controls.
- Elevated systemic endotoxin correlated with all clinical parameters and most measured GCF cyto/chemokines.
Conclusions:
- LAP is associated with significantly higher systemic endotoxin levels.
- Systemic endotoxin correlates with an amplified local inflammatory response in the gingiva.
- Findings highlight the link between systemic endotoxemia and the pathogenesis of localized aggressive periodontitis.
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