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Published on: June 16, 2020
Isolation and characterization of microsatellite loci from apomictic Hypericum perforatum (Hypericaceae)
Jose M Corral1, Marta Puente Molins, Olawale M Aliyu
1Apomixis Research Group, Department of Cytogenetics and Genome Analysis, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, Gatersleben, Germany 06466. corral@ipk-gatersleben.de
Premise Of The Study:
Microsatellite markers were developed to study the origins and evolution of apomictic Hypericum perforatum, a polyploid invasive perennial herb indigenous to Europe that produces compounds of medicinal/pharmaceutical importance.
Methods And Results:
Eleven primer sets were developed using a non-radioactive protocol. All loci were polymorphic, showing from 2 to 9 alleles per locus. Observed and expected heterozygosity averaged 0.861 and 0.772, respectively, across four studied populations. Most primers also amplified successfully in eight other Hypericum species.
Conclusions:
Markers developed in this study provide genetic tools for studies of apomixis and invasiveness, both on the intra- and interspecific levels.
