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Published on: March 27, 2020

YueF overexpression inhibits cell proliferation partly through p21 upregulation in renal cell carcinoma

Hsuan-Wei Huang1, Jian-Ping Peng, Jie Zhang

  • 1Department of Urology, RenMin Hospital of Wuhan University, Wuhan 430060, China; E-Mails: xuanwei_huang@163.com (H.-W.H.); jianping0209@163.com (J.-P.P.).

International Journal of Molecular Sciences
|July 7, 2011
PubMed

Insights

YueF, a tumor suppressor gene, is downregulated in renal cell carcinoma (RCC). Overexpressing YueF inhibits RCC cell proliferation by inducing cell cycle arrest and upregulating p53 and p21(WAF1/Cip1).

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • YueF is a novel putative tumor suppressor gene.
  • Its role in renal cell carcinoma (RCC) is currently unknown.

Purpose of the Study:

  • To investigate YueF gene expression in RCC tissues.
  • To determine the effect of YueF on RCC cell proliferation.

Main Methods:

  • Examined YueF expression in RCC tissues and 786-0 cells.
  • Utilized lentivirus-mediated YueF overexpression in 786-0 cells.
  • Analyzed cell cycle, proliferation, and protein levels (p53, p21(WAF1/Cip1), cyclin D1, pRb).

Main Results:

  • YueF expression was reduced or absent in RCC tissues and 786-0 cells compared to normal kidney tissues.
  • YueF overexpression induced G1 phase cell-cycle arrest and reduced proliferation in 786-0 cells.
  • YueF increased p53 and p21(WAF1/Cip1) levels, while decreasing cyclin D1 and pRb levels.

Conclusions:

  • YueF acts as a tumor suppressor in RCC.
  • YueF-induced growth inhibition in 786-0 cells is mediated, in part, by p21.
  • Findings offer new insights into YueF's tumor-suppressive mechanisms in RCC.
Keywords:
YueFclear cell RCCp21p53

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