Related Experiment Video
Updated: May 31, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
Published on: February 10, 2020
Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss
Delong Zhang1, Mikhail N Slipchenko, Ji-Xin Cheng
1Department of Chemistry, Purdue University, West Lafayette, IN 47907.
Abstract:
Nonlinear vibrational imaging of live cells and organisms is demonstrated by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produced a 12 times larger stimulated Raman loss signal than picosecond pulse excitation. The large signal allowed real-time imaging of the conversion of deuterated palmitic acid into lipid droplets inside live cells, and three-dimensional sectioning of fat storage in live C. elegans. With the majority of the excitation power contributed by the Stokes beam in the 1.0 to 1.2 microm wavelength range, photodamage of biological samples was not observed.
Related Concept Videos
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Super-resolution Fluorescence Microscopy
