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Updated: May 31, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Thiolene-based microfluidic flow cells for surface plasmon resonance imaging.

Gareth Sheppard, Takao Oseki, Akira Baba

    Biomicrofluidics
    |July 7, 2011
    PubMed
    Summary
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    This study integrates thiolene microfluidics with surface plasmon resonance imaging for studying interfacial interactions. The developed devices enable sensitive nanomolar detection in various solutions, showcasing their versatility.

    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Materials Science

    Background:

    • Surface Plasmon Resonance Imaging (SPRI) is a label-free optical technique for studying molecular interactions at surfaces.
    • Microfluidic devices offer precise control over small fluid volumes and reaction conditions.
    • Integrating microfluidics with SPRI enhances the sensitivity and applicability of interfacial interaction studies.

    Purpose of the Study:

    • To develop an integrated microfluidic-SPRI platform using thiolene-based devices.
    • To enable the study of interfacial interactions in both aqueous and organic solutions.
    • To demonstrate the platform's capability for sensitive molecular detection.

    Main Methods:

    • A photolithographic method was employed to fabricate thiolene microfluidic channels on gold and glass substrates.

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  • The microfluidic channels were designed for excellent adhesion, preserving sensor surface integrity.
  • The integrated system was utilized for in situ Surface Plasmon Resonance Imaging (SPRI) measurements.

    • Main Results:

    • The thiolene-based microfluidic devices exhibited excellent adhesion and compatibility with various solvents, including organic solvents.
    • The devices sustained high flow rates, crucial for efficient interaction studies.
    • Nanomolar detection of streptavidin-biotin interactions was successfully achieved using the integrated platform.

    Conclusions:

    • The developed thiolene-based microfluidic-SPRI platform provides a versatile and robust tool for studying interfacial interactions.
    • The fabrication method ensures sensor surface accessibility for modification and reliable measurements.
    • This integrated approach facilitates sensitive detection of molecular binding events in diverse solution environments.