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Updated: May 31, 2026

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Published on: December 24, 2015

CD73 and CD29 concurrently mediate the mechanically induced decrease of migratory capacity of mesenchymal stromal

A Ode¹, J Kopf, A Kurtz

¹Julius Wolff Institute and Musculoskeletal Research Center Berlin, Charité-Universitätsmedizin, Berlin, Germany.

European Cells & Materials

|July 7, 2011

Summary

Mechanical loading reduces mesenchymal stromal cell (MSC) migration by decreasing CD73 and CD29 expression. This impaired migration, crucial for bone repair, persists even after mechanical stimulus removal, impacting regenerative therapies.

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Last Updated: May 31, 2026

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

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Published on: December 24, 2015

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Published on: January 7, 2015

Area of Science:

Biomedical Engineering
Cell Biology
Regenerative Medicine

Background:

Mesenchymal stromal cell (MSC)-based therapies aim to enhance natural regeneration.
The mechanical microenvironment significantly influences MSC behavior during regeneration, especially in bone fracture repair.
Understanding MSC response to biomechanical stimulation is critical for therapeutic success.

Purpose of the Study:

To investigate how mechanical loading affects MSC characteristics and function.
To elucidate the role of MSC surface markers CD73 and CD29 in response to mechanical stimuli.
To identify downstream pathways regulating MSC migration under mechanical stress.

Main Methods:

Mechanical loading applied to MSCs.
Analysis of MSC surface marker expression (CD73, CD29) via quantitative methods.
Assessment of MSC migration capacity.
Inhibition studies of CD73/CD29.
Microscopy (SEM) and phalloidin staining to evaluate cell morphology and actin cytoskeleton.
Investigation of focal adhesion kinase (FAK) and Src-family kinases.

Main Results:

Mechanical loading reduced CD73 and CD29 expression on MSCs.
MSC migration was significantly decreased after mechanical loading, with effects lasting up to a week post-stimulus.
CD73/CD29 were confirmed to be involved in MSC migration post-loading.
Reduced cell spreading, lamellipodia formation, and actin accumulation observed.
FAK and Src-family kinases identified as potential downstream targets.

Conclusions:

Mechanical stimulation negatively regulates MSC migration through modulation of CD73/CD29 expression.
These surface markers are key regulators of MSC migration in response to mechanical cues.
The findings suggest a mechanism where MSCs become mechanically entrapped at fracture sites, facilitating regeneration.