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A complement-dependent neutralizing monoclonal antibody against glycoprotein II of pseudorabies virus
T Nakamura1, T Ihara, T Nagata
1Nippon Institute for Biological Science, Tokyo, Japan.
Veterinary Microbiology
|August 1, 1990
Abstract:
A monoclonal antibody (MAb), 1.21, was produced against pseudorabies virus (PRV). It exhibited virus neutralization activity only in the presence of complement. Immunoblot analysis after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of virions revealed that MAb 1.21 bound with the 230 kilodalton (kD) virus protein only under non-reducing conditions. This protein was purified by immuno-affinity chromatography using MAb 1.21 and was found to be composed of three subunits, 60, 70 and 120 kD polypeptides when analyzed by SDS-PAGE under reducing conditions. This protein is probably glycoprotein II of PRV.