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Updated: May 31, 2026
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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
Published on: March 24, 2023
CD34+ cell enumeration by flow cytometry: a comparison of systems and methodologies
Alain Ngoma1, Shunnichi Saito, Hitoshi Ohto
1Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima City, Fukushima, Japan.
Archives of Pathology & Laboratory Medicine
|July 8, 2011
Summary
Comparing two flow cytometry systems for CD34(+) cell counting, this study found subtle differences between single-platform and dual-platform methods that were not statistically significant. These validated methods support patient care and multicenter research.
Area of Science:
- Hematology
- Immunophenotyping
- Flow Cytometry
Background:
- Increasing stem cell collections necessitate laboratory adaptations.
- Investment in new equipment for pathology labs may be impractical.
Purpose of the Study:
- To compare CD34(+) cell counting using two flow cytometry systems.
- To evaluate single-platform versus dual-platform methodologies for cell enumeration.
Main Methods:
- Analysis of whole blood and peripheral blood stem cell (PBSC) samples.
- Comparison of Cytomics FC500 and FACSCalibur flow cytometry systems.
- Inclusion of data from a KX-21 hematology analyzer for dual-platform analysis.
Main Results:
- No statistically significant differences in CD34(+) cell counts were observed between the two systems.
- Subtle variations between single-platform and dual-platform methods did not reach statistical significance.
Conclusions:
- Validated flow cytometry systems and methods for CD34(+) cell enumeration can support patient care.
- Consistent data across different platforms are valuable for multicenter studies and meta-analyses.
