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KPC screening by updated BD Phoenix and Vitek 2 automated systems
Kenneth S Thomson1, Iraida E Robledo, Guillermo J Vázquez
1Center for Research in Antiinfectives & Biotechnology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178-0404, USA. kstaac@creighton.edu
Journal of Clinical Microbiology
|July 8, 2011
Summary
BD Phoenix and Vitek 2 systems effectively screen for carbapenemase-producing Klebsiella pneumoniae (KPC) beta-lactamases. These methods show high sensitivity for detecting KPC-producing Gram-negative bacteria, aiding in infection control.
Area of Science:
- Clinical Microbiology
- Antimicrobial Resistance
- Infectious Diseases
Background:
- Carbapenemase-producing Klebsiella pneumoniae (KPC) are a significant public health threat.
- Rapid detection of KPC is crucial for effective patient management and infection control.
Purpose of the Study:
- To evaluate the performance of BD Phoenix and Vitek 2 systems as screening tools for KPC beta-lactamases.
- To determine the sensitivity of these automated systems in identifying KPC-producing Gram-negative bacteria.
Main Methods:
- Assessment of BD Phoenix and Vitek 2 methodologies.
- Utilized carbapenem Minimum Inhibitory Concentrations (MICs) and expert system interpretations as screening criteria.
- Tested against 103 well-characterized Gram-negative bacterial isolates.
Main Results:
- Both BD Phoenix and Vitek 2 systems demonstrated high sensitivity (97%) for KPC detection.
- The study included 77 isolates confirmed as KPC producers.
- Carbapenem MICs and expert system interpretations proved effective as screening parameters.
Conclusions:
- BD Phoenix and Vitek 2 systems are reliable and sensitive tools for screening KPC beta-lactamases in clinical laboratories.
- These automated systems can aid in the early identification of Gram-negative bacteria producing KPC, facilitating timely interventions.
