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Updated: May 31, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Real-time detection of blaKPC in clinical samples and surveillance specimens
Kathy A Mangold1, Kristine Santiano, Ronit Broekman
1Pathology & Laboratory Medicine, NorthShore University Health System, 2650 Ridge Avenue, Evanston, IL 60201, USA. kmangold@northshore.org
Abstract:
A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity.
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