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Updated: May 31, 2026

Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein
Published on: July 5, 2024
[Functions of plant apyrases]
Magdalena Wujak1, Michał Komoszyński
1Instytut Biologii Ogólnej i Molekularnej, Zakład Biochemii Uniwersytetu Mikołaja Kopernika, ul. Gagarina 7, 87-100 Toruń.
Plant nucleoside tri-/diphosphatases (NTPDases), or apyrases, are crucial enzymes involved in nucleotide metabolism. These enzymes regulate cellular energy and are vital for plant growth, development, and symbiotic interactions.
Area of Science:
- Biochemistry
- Plant Physiology
Context:
- Plant nucleoside tri-/diphosphatases (NTPDases), also known as apyrases, are enzymes that hydrolyze nucleoside tri- and di-phosphates.
- These enzymes are found in various cellular compartments, existing as soluble or membrane-bound proteins.
Purpose:
- To review the structure, physicochemical properties, and metabolic functions of plant NTPDases.
- To explore the roles of apyrases in Solanum tuberosum and Arabidopsis thaliana.
Summary:
- Plant NTPDases exhibit conserved structural features, broad substrate specificity, and require divalent cations for activity.
- Apoplastic NTPDases produce adenine, ribose, and orthophosphate, which are transported into cells.
- These enzymes are implicated in regulating starch synthesis, signal transmission, and are essential for tuber and root development.
Impact:
- NTPDases play a critical role in regulating cellular energy charge by altering nucleotide concentrations.
- They are essential for symbiotic relationships, such as those between leguminous plants and root nodule bacteria.
- Understanding plant NTPDases provides insights into fundamental plant biological processes and agricultural applications.
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