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[Cleaning of Sendai virus-infected mice by embryo transfer technique]
M Okamoto1, S Matsushita, T Matsumoto
1National Institute of Radiological Sciences, Chiba-shi, Japan.
Abstract:
Mouse embryos (8-16 cells) were collected from Sendai virus (SV)-infected mice at 5 or 7 weeks after inoculation. All donors having embryo(s) were positive when tested by CF test or ELISA for SV antibody at the time of embryo collection. Most of the morphologically normal embryos developed (90.6%, 259/286) to morulae or blastocysts after culture for 26-28 hr. A total of 76 embryos cultured were transferred to the uteri of SV-free pseudopregnant recipients. Forty-seven young were obtained from these recipients (61.8% of development rate) and 46 young were successfully reared up to 10th week of age. All the recipients and the young were negative by testing SV antibody. These results indicate that the embryo transfer technique is useful for cleaning of SV-infected mice.
Insights
Sendai virus (SV) infection in mice can be cleared using embryo transfer techniques. This method produced healthy, SV-antibody-negative offspring from infected mothers, demonstrating its effectiveness for disease eradication.
Area of Science:
- Reproductive biology
- Virology
- Animal science
Context:
- Sendai virus (SV) poses a significant challenge in research animal colonies.
- Maternal transmission of SV can compromise experimental integrity.
- Effective methods for eradicating SV from infected mouse colonies are crucial.
Purpose:
- To evaluate the efficacy of embryo transfer for eliminating Sendai virus from infected mouse populations.
- To assess the health and viral status of offspring derived from SV-infected dams via embryo transfer.
Summary:
- Mouse embryos (8-16 cell stage) were collected from SV-infected dams.
- Morphologically normal embryos developed successfully to morulae/blastocysts in vitro.
- Embryo transfer to SV-free recipients resulted in healthy, SV-antibody-negative offspring.
Impact:
- Embryo transfer is a viable strategy for producing virus-free mice.
- This technique aids in maintaining disease-free research animal colonies.
- Contributes to the control and eradication of viral infections in laboratory animals.