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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Protein alpha-N-acetylation studied by N-terminomics.

Petra Van Damme¹, Thomas Arnesen, Kris Gevaert

¹Department of Medical Protein Research, VIB, Ghent, Belgium. petra.vandamme@vib-ugent.be

The FEBS Journal

|July 9, 2011

Summary

Protein N-terminal acetylation, a universal cotranslational modification, impacts protein function. Emerging N-terminomics and proteogenomics technologies are advancing our understanding of this vital biological process.

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Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Cotranslational protein N-terminal modifications, like methionine excision and N-terminal blocking, are universal biological processes.
Protein alpha-N-acetylation, catalyzed by N-terminal acetyltransferase complexes, occurs during translation and influences protein folding, stability, activity, and localization.
Historically, protein alpha-N-acetylation was under-explored due to a lack of targeted proteomics technologies.

Purpose of the Study:

To review emerging N-terminomics and proteogenomics technologies.
To highlight recent breakthroughs in understanding protein N-terminal biology, particularly alpha-N-acetylation.
To underscore the importance of protein N-terminal modifications.

Main Methods:

N-terminomics technologies for isolating protein N-terminal peptides.
Proteogenomics efforts integrating experimental and informational data.
Review of existing literature on protein modifications and N-terminal biology.

Main Results:

The emergence of N-terminomics and proteogenomics has significantly advanced the study of protein alpha-N-acetylation.
These technologies enable detailed analysis of protein N-terminal peptides.
Significant progress has been made in understanding the functions and regulation of protein N-terminal modifications.

Conclusions:

Protein N-terminal acetylation is a crucial modification with broad functional implications.
Advanced technologies are revolutionizing the study of protein N-terminal biology.
Further research in N-terminomics and proteogenomics promises deeper insights into protein regulation.