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Localisation of human papillomavirus 16 E7 oncoprotein changes with cell confluence
1Division of Virology, National Institute for Medical Research, Medical Research Council, London, United Kingdom. Joanna.Laurson@doube.net
Plos One
|July 9, 2011
Summary
The location of human papillomavirus type 16 E7 protein shifts from the nucleus and cytoplasm to the cytoplasm in confluent cells. This change, along with increased E7 levels, is independent of cell cycle or differentiation.
Area of Science:
- Oncology
- Virology
- Cell Biology
Background:
- Human papillomavirus type 16 (HPV16) E7 protein is crucial for cervical cancer development.
- The sub-cellular localization of E7 has been difficult to ascertain, despite its known shuttling between the nucleus and cytoplasm.
Purpose of the Study:
- To investigate the sub-cellular localization of HPV16 E7 protein.
- To determine factors influencing E7 localization and expression.
- To highlight the impact of analytical techniques on localization studies.
Main Methods:
- Immunocytochemistry was used to visualize E7 protein localization.
- Western blotting was employed to quantify E7 protein levels.
- Cells were analyzed under sub-confluent and confluent conditions.
Main Results:
- E7 protein localized to both nucleus and cytoplasm in sub-confluent cells.
- E7 predominantly localized to the cytoplasm in confluent cells.
- E7 levels increased in confluent cells, independent of cell cycle or differentiation.
- Different analytical techniques influenced E7 localization observations.
Conclusions:
- Cellular confluency induces a significant shift in E7 sub-cellular localization.
- Understanding E7 localization is key to comprehending its function and oncogenic potential.
- Methodological choices critically affect the interpretation of E7 localization data.
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