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Calcium/calmodulin-dependent protein kinase II.

R J Colbran1, T R Soderling

  • 1Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

Current Topics in Cellular Regulation
|January 1, 1990
PubMed
Summary

Calcium/calmodulin-dependent protein kinase II (CaM kinase II) is well-characterized in vitro, but its in vivo functions remain largely unknown. Future research will focus on its physiological roles in the brain and other tissues.

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Area of Science:

  • Biochemistry
  • Neuroscience
  • Molecular Biology

Background:

  • CaM kinase II exhibits well-defined in vitro properties regarding substrate specificity and regulation by calmodulin and autophosphorylation.
  • Studies have primarily utilized the rat brain isozyme, with other tissue isozymes showing similar in vitro behavior.
  • Significant gaps exist in understanding the kinase's in vivo functions and the physiological relevance of its autophosphorylation.

Purpose of the Study:

  • To highlight the current understanding of CaM kinase II's in vitro characteristics.
  • To identify the limitations in current knowledge regarding the kinase's in vivo functions.
  • To emphasize the need for future research into the physiological roles of CaM kinase II.

Main Methods:

  • In vitro biochemical assays to characterize substrate specificity and regulatory mechanisms.
  • Comparative analysis of isozymes from different tissues.
  • Review of existing literature on CaM kinase II function.

Main Results:

  • Extensive characterization of CaM kinase II's in vitro properties, including substrate interactions and regulatory pathways.
  • Demonstration of broadly similar in vitro behavior across different tissue isozymes.
  • Identification of unknown in vivo substrates and the role of autophosphorylation.

Conclusions:

  • While CaM kinase II is well-understood in vitro, its in vivo functions are largely unexplored.
  • Investigating the physiological roles of CaM kinase II in vivo presents a significant and exciting area for future research.
  • Availability of cDNA clones and in vitro data should facilitate future in vivo investigations.

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