Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yellow Fever01:18

Yellow Fever

Yellow fever is a viral hemorrhagic disease caused by the yellow fever virus (YFV), a member of the Flaviviridae family. It is transmitted primarily by Aedes and Haemagogus mosquitoes in tropical and subtropical regions of Africa and South America. After transmission through a mosquito bite, the virus initially replicates in skin-resident immune cells such as dendritic cells and macrophages. These cells then migrate to the lymph nodes, where viral replication increases, eventually leading to...
Lethal Alleles02:41

Lethal Alleles

Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Changes in Skin Color: Clinical Perspectives01:14

Changes in Skin Color: Clinical Perspectives

The first thing a clinician sees is the skin, so the examination of the skin should be part of any thorough physical examination. Most skin disorders are relatively benign, but a few, including melanomas, can be fatal if untreated. A couple of the more noticeable disorders, albinism and vitiligo, affect the appearance of the skin and its accessory organs.
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Lucid Dreaming01:10

Lucid Dreaming

Lucid dreaming is a unique state of consciousness where an individual realizes they are dreaming while still in the dream. This awareness allows them to manipulate their dream environment consciously. Researchers like Stephen LaBerge have significantly contributed to the understanding of lucid dreams, highlighting that during these dreams, certain areas of the brain, such as the prefrontal cortex, that involve self-awareness and thought evaluation show increased activity.
Studies have shown...
Dark Triad and Person Perception01:29

Dark Triad and Person Perception

Person perception is influenced by both external behaviors and the observer’s internal characteristics, including personality traits. Individuals with dark personality traits, comprising psychopathy, Machiavellianism, and narcissism — collectively known as the dark triad – exhibit manipulative and exploitative tendencies in social contexts. These traits affect how they perceive others and how they are perceived.The Role of Dark Personality Traits in Person PerceptionBlack et al. (2014) explored...
Pigmentation01:19

Pigmentation

The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum from Fibromyalgia Patients Activates Satellite Glial Cells in Mouse Peripheral Ganglia.

Cells·2026
Same author

Ennio Pannese (1928-2025).

Neuron·2026
Same author

Demyelination-related pain: role of lysophosphatidic acid in satellite glial cell-neuron crosstalk.

Journal of translational medicine·2025
Same author

Meteorin Resolves Nociceptive Hypersensitivity by Reducing Connexin-Mediated Coupling in Satellite Glial Cells.

Glia·2025
Same author

Conserved small RNA networks link inflammation to pain signaling in mice and men.

Translational psychiatry·2025
Same author

Expansion Microscopy of the Enteric Nervous System: A Feasibility Study.

Cells·2025

Related Experiment Video

Updated: May 31, 2026

Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
06:22

Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier

Published on: August 19, 2019

Lucifer yellow - an angel rather than the devil.

Menachem Hanani1

  • 1Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem, Israel. hananim@cc.huji.ac.il

Journal of Cellular and Molecular Medicine
|July 12, 2011
PubMed
Summary

Lucifer yellow (LY) dye, introduced in 1978, is vital for cell biology research. It

Area of Science:

  • Cell biology
  • Neuroscience
  • Histology

Background:

  • Lucifer yellow (LY) is a fluorescent dye established in 1978.
  • It is widely utilized for investigating cellular structure and intercellular communication.
  • Intracellular injection via microelectrodes is the primary method for cell labeling with LY.

Purpose of the Study:

  • To review the diverse applications of Lucifer yellow.
  • To highlight LY's utility in studying the enteric nervous system.
  • To emphasize LY's role in examining interstitial cells of Cajal.

Main Methods:

  • Intracellular injection of Lucifer yellow dye.
  • Microelectrode techniques for dye delivery.
  • Dye coupling method for gap junction analysis.

More Related Videos

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
07:38

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Published on: September 14, 2019

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
09:48

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Published on: July 27, 2022

Related Experiment Videos

Last Updated: May 31, 2026

Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
06:22

Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier

Published on: August 19, 2019

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
07:38

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Published on: September 14, 2019

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
09:48

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Published on: July 27, 2022

Main Results:

  • Lucifer yellow facilitates detailed cell structure visualization.
  • It enables the study of cell-to-cell communication pathways.
  • The dye coupling method effectively detects gap junction-mediated cell coupling.

Conclusions:

  • Lucifer yellow remains an indispensable tool in cell biology.
  • Its application extends to specialized systems like the enteric nervous system.
  • LY is crucial for understanding gap junction function through dye coupling.