Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In multi electron beam systems, "Neighbours Matter".

Ultramicroscopy·2023
Same author

Electron-beam patterned calibration structures for structured illumination microscopy.

Scientific reports·2022
Same author

Principles of electron wave front modulation with two miniature electron mirrors.

Ultramicroscopy·2021
Same author

Combined Focused Electron Beam-Induced Deposition and Etching for the Patterning of Dense Lines without Interconnecting Material.

Micromachines·2020
Same author

Flat electron mirror.

Ultramicroscopy·2020
Same author

Pulse length, energy spread, and temporal evolution of electron pulses generated with an ultrafast beam blanker.

Structural dynamics (Melville, N.Y.)·2019

Related Experiment Video

Updated: May 31, 2026

Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry
07:24

Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry

Published on: May 6, 2019

Simulation of ion imaging: sputtering, contrast, noise.

V Castaldo1, C W Hagen, P Kruit

  • 1Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands. v.castaldo@tudelft.nl

Ultramicroscopy
|July 12, 2011
PubMed
Summary

Scanning ion microscopy (SIM) image interpretation is complex. A new simulation approach considers sputtering, contrast, and signal-to-noise ratio for better analysis of ion imaging tools.

More Related Videos

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry
07:10

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry

Published on: April 29, 2020

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
10:22

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

Related Experiment Videos

Last Updated: May 31, 2026

Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry
07:24

Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry

Published on: May 6, 2019

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry
07:10

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry

Published on: April 29, 2020

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
10:22

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

Area of Science:

  • * Physics
  • * Materials Science
  • * Surface Science

Background:

  • * Scanning ion microscopy (SIM) has advanced with new light and inert gas ion sources.
  • * Interpreting SIM images is challenging due to different contrast mechanisms and sample sputtering.
  • * Existing Monte Carlo methods for simulating ion-sample interactions are computationally intensive.

Purpose of the Study:

  • * To develop a novel simulation approach for scanning ion microscopy imaging.
  • * To provide a more comprehensive method for evaluating SIM tools beyond single parameters.
  • * To improve the understanding of contrast, sputtering, and noise in SIM.

Main Methods:

  • * Simulation of image contrast using secondary electron yield curves versus incidence angle.
  • * Prediction of surface modification via sputtering yield curves.
  • * Incorporation of Poisson noise from primary ions and secondary electrons.
  • * Addition of detector noise contributions.

Main Results:

  • * Proposed simulation approach shows good agreement with experimental data for certain imaging aspects.
  • * Evaluation of SIM tools requires analysis of sputtering, contrast, and signal-to-noise ratio.
  • * Detector noise can be a limiting factor in focused ion beam imaging.

Conclusions:

  • * The proposed modular simulation approach offers a more robust evaluation of SIM performance.
  • * Further effects like edge enhancement can be integrated into the simulation framework.
  • * This method aids in optimizing SIM techniques and interpreting complex ion images.