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Updated: May 31, 2026

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Live Cell Imaging with Time Lapse Photography to Study Epidermal Keratinocyte Proliferation Kinetics
Published on: June 6, 2025
Characterization of spatial cell distribution in multilayer sheet of human keratinocytes through a stereoscopic cell
Mee-Hae Kim1, Naoki Tsubakino, Shogo Kagita
1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, Japan.
Journal of Bioscience and Bioengineering
|July 12, 2011
Abstract:
A novel system was developed to characterize the spatial distribution of human keratinocytes in cultured epithelial sheet, based on stereoscopic observation of nuclei using a confocal laser microscope in combination with image processing. The presented technique allowed understanding of the proliferative and growth-arrested states of cells in multilayered sheet.

