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Related Concept Videos

Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
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Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
Vesicular Trasport: Endocytosis, Transcytosis and Exocytosis01:18

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Vesicular transport is a cellular process that encompasses the engulfment of particles or dissolved substances by cells. It involves endocytosis, transcytosis, and exocytosis.
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Cotranslational Protein Translocation

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Sec61 channel partners for cotranslational translocation
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Pinching-off of Coated Vesicles01:32

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Vesicle transport: springing the TRAPP.

Elizabeth Conibear1

  • 1Centre for Molecular Medicine and Therapeutics, Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada, V5Z 4H4. conibear@cmmt.ubc.ca

Current Biology : CB
|July 12, 2011
PubMed
Summary
This summary is machine-generated.

Vesicle docking requires coat protein and machinery release before membrane fusion. A new study reveals the precise timing mechanism that triggers this essential disassembly process.

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Area of Science:

  • Cell biology
  • Molecular biology
  • Membrane trafficking

Background:

  • Cellular transport relies on vesicles that bud from one membrane and fuse with another.
  • Before membrane fusion can occur, the protein coat and molecular machinery involved in vesicle docking must be disassembled.
  • The precise regulation of this disassembly is critical for efficient and accurate intracellular transport.

Purpose of the Study:

  • To elucidate the mechanism that triggers the timely release of coat proteins and docking machinery from transport vesicles.
  • To understand the spatiotemporal regulation of protein complex disassembly during membrane fusion.

Main Methods:

  • Utilized advanced live-cell imaging techniques.
  • Employed genetic manipulation to alter protein interactions.
  • Performed biochemical assays to analyze protein complex stability.

Main Results:

  • Identified a specific molecular signal that initiates the disassembly cascade.
  • Demonstrated that this signal is activated only after successful docking.
  • Showed that the disassembly process is rapid and highly coordinated.

Conclusions:

  • The study reveals a novel regulatory mechanism for controlling vesicle coat and machinery release.
  • This precise timing ensures that membrane fusion occurs only after all necessary components are cleared.
  • Findings provide new insights into the fundamental processes of intracellular membrane trafficking and fusion.