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Human chorionic gonadotropin from day 2 spent embryo culture media and its relationship to embryo development
Sivakumar Ramu1, Brian Acacio, Mark Adamowicz
1Andrology Laboratory Services Inc., Chicago, Illinois 60611, USA. siva@androlab.com
Objective:
To detect hCG in spent embryo culture media at day 2 after intracytoplasmic sperm injection and to assess the relationship of hCG to embryo development.
Design:
Experimental study.
Setting:
Fertility center and clinical diagnostic laboratory.
Sample(S):
A total of 102 spent culture media from day 2 human embryos and corresponding unexposed media for blank control.
Intervention(S):
The culture media samples were tested for hCG by ELISA.
Main Outcome Measure(S):
Quantity of hCG produced by embryos and correlation with the embryos' developmental status.
Result(S):
hCG was found in 93 of 102 culture media tested by enzyme-linked immunosorbent assay. The correlation analysis revealed that the concentration of hCG was independent of embryo developmental status.
Conclusion(S):
The ability to detect hCG from day 2 spent culture media may be used as a marker for embryo competence.
