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Ca channel gating during cardiac action potentials.

M Mazzanti1, L J DeFelice

  • 1Department of Anatomy and Cell Biology, Emory University, Atlanta, Georgia 30322.

Biophysical Journal
|October 1, 1990
PubMed
Summary
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Calcium (Ca) channels conduct Ca ions during cardiac action potentials. Rare, long-opening Ca channel events, though infrequent, may significantly contribute to total Ca ion influx.

Area of Science:

  • Cardiovascular Physiology
  • Ion Channel Biophysics
  • Cardiac Electrophysiology

Background:

  • Understanding calcium (Ca) ion conduction through cardiac channels during the action potential is crucial for cardiac function.
  • Previous studies utilized a two-microelectrode technique for Na and K currents, but its application to Ca channels requires further investigation.

Purpose of the Study:

  • To investigate how Ca channels conduct Ca ions during the cardiac action potential.
  • To compare action currents from the two-microelectrode technique with standard voltage-clamp experiments.

Main Methods:

  • Employed a two-microelectrode technique to simultaneously record patch current and action potential.
  • Measured individual Ca channel activity using 10 mM Ca/1 Ba and 10 mM Ba as charge carriers.

Related Experiment Videos

  • Compared results with step-protocol currents from voltage-clamp experiments.

    • Main Results:

    • With Ba as the charge carrier, Ca channel kinetics showed voltage-dependent conversion from long to short open times between -20 and 20 mV.
    • With Ca as the charge carrier, currents were brief; however, rare spontaneous conversions to long-open-time kinetics (mode 2) were observed.
    • In rare mode 2 events, single channel conductance in 10 Ca/1 Ba was measured at 8-10 pS, approximately half that in Ba.

    Conclusions:

    • Infrequent long openings of Ca channels with Ca as the charge carrier contribute negligibly to average instantaneous Ca current.
    • Despite their rarity, these long openings may significantly contribute to the total Ca ion influx during the cardiac action potential.
    • The observed voltage-dependent kinetics with Ba provide insights into Ca channel behavior under different conditions.