Related Experiment Video

Updated: May 31, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Whole-genome sequence of a multidrug-resistant clinical isolate of Acinetobacter lwoffii

Yongfei Hu1, Wei Zhang, Hui Liang

  • 1CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People’s Republic of China.

Journal of Bacteriology
|July 12, 2011
PubMed

Insights

We sequenced the genome of Acinetobacter lwoffii WJ10621, a multidrug-resistant bacterium. This isolate harbors a plasmid containing the New Delhi metallo-beta-lactamase 1 (NDM-1) gene, contributing to its resistance.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Acinetobacter lwoffii is recognized as an opportunistic pathogen.
  • Nosocomial infections caused by A. lwoffii pose a significant threat in healthcare settings.

Purpose of the Study:

  • To present the complete genome sequence of a multidrug-resistant Acinetobacter lwoffii clinical isolate, designated WJ10621.
  • To identify genetic elements, such as plasmids, that confer antimicrobial resistance.

Main Methods:

  • Whole-genome sequencing of the Acinetobacter lwoffii WJ10621 isolate.
  • Bioinformatic analysis to identify genes and mobile genetic elements, including plasmids and resistance genes.

Main Results:

  • The genome sequence of A. lwoffii WJ10621 was successfully obtained.
  • A plasmid carrying the New Delhi metallo-beta-lactamase 1 (NDM-1) resistance gene was identified within the isolate.

Conclusions:

  • The genome sequence provides valuable insights into the genetic makeup of this multidrug-resistant A. lwoffii strain.
  • The presence of the NDM-1 gene highlights the potential for rapid dissemination of antimicrobial resistance in clinical settings.

More Related Videos

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System05:06

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Related Concept Videos

Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Antibiotic Selection00:57

Antibiotic Selection

Overview