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Related Experiment Video

Updated: May 31, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

Lessons from morpholino-based screening in zebrafish.

Victoria M Bedell1, Stephanie E Westcot, Stephen C Ekker

  • 1Mayo Clinic, Guggenheim, Rochester, MN 55905, USA.

Briefings in Functional Genomics
|July 13, 2011
PubMed
Summary
This summary is machine-generated.

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Morpholino oligonucleotides (MOs) offer effective gene knockdown in zebrafish for functional gene analysis. Careful management ensures MOs remain valuable for screening and individual gene studies.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Morpholino oligonucleotides (MOs) are established antisense agents for gene knockdown.
  • Zebrafish (Danio rerio) is a widely used model organism for in vivo studies.

Purpose of the Study:

  • To detail the application of MOs in zebrafish for in vivo gene function characterization.
  • To provide guidance on MO screening, data management, and off-target effect control.

Main Methods:

  • Gene target selection and screening parameter considerations.
  • In vivo functional characterization using MOs in zebrafish.
  • Management of off-target effects and downstream phenotypic validation.

Main Results:

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Published on: March 7, 2025

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
07:45

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

Related Experiment Videos

Last Updated: May 31, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae
02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
07:45

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

  • MOs enable effective gene-specific knockdown for functional analysis in zebrafish.
  • Systematic screening, data management, and validation are crucial for reliable results.
  • Advances offer spatiotemporal control, enhancing MO utility.

Conclusions:

  • MOs are a valuable tool for discovery screening and individual gene knockdown in zebrafish.
  • Careful management, including off-target effect control, is essential for successful application.
  • Ongoing technological advancements continue to improve MO-based research capabilities.