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Related Experiment Video

Updated: May 31, 2026

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA₂

Published on: September 26, 2025

Secreted phospholipase A2 revisited.

Makoto Murakami¹, Yoshitaka Taketomi, Hiroyasu Sato

¹Lipid Metabolism Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.

Journal of Biochemistry

|July 13, 2011

Summary

Secreted phospholipase A(2) (sPLA(2)) enzymes, once obscure, are now understood for their specific roles in pathophysiology. Recent research clarifies their expression, function, and extracellular actions.

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A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA₂

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Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

Area of Science:

Biochemistry
Enzymology
Molecular Biology

Background:

Phospholipase A(2) (PLA(2)) enzymes hydrolyze glycerophospholipids at the sn-2 position.
Mammals possess over 30 PLA(2) enzymes, including the secreted PLA(2) (sPLA(2)) family.
sPLA(2)s are low molecular weight, Ca(2+)-dependent enzymes with distinct localizations and functions.

Purpose of the Study:

To review recent advances in PLA(2) research, focusing on sPLA(2)s.
To elucidate the physiological functions and modes of action of sPLA(2)s.
To highlight extracellular phospholipid targets and lipid mediator production by sPLA(2)s.

Main Methods:

Studies utilizing knockout and transgenic mice.
Application of specific PLA(2) inhibitors.
Lipidomics analysis for isoform expression and function.

Main Results:

Clarification of sPLA(2) isoform expression patterns in vivo.
Identification of sPLA(2) involvement in specific pathophysiological conditions.
Understanding of sPLA(2) mechanisms in extracellular environments.

Conclusions:

The biological roles of sPLA(2)s in vivo are increasingly understood.
sPLA(2)s exhibit specialized functions in various pathophysiological contexts.
Research has advanced our knowledge of sPLA(2)s' extracellular targets and mediator production.