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Updated: May 31, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Multicenter evaluation of a new DNA microarray for rapid detection of clinically relevant bla genes from
Pierre Bogaerts1, Andrea M Hujer, Thierry Naas
1Laboratoire de Bactériologie, Cliniques Universitaires UCL de Mont-Godinne, Av Dr Therasse 1, B-5530 Yvoir, Belgium. pierre.bogaerts@uclouvain.be
Abstract:
A new commercial low-density microarray which identifies common extended-spectrum β-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (bla(KPC) and bla(NDM)) genes, was evaluated. We tested 207 clinical and reference/collection isolates of the Enterobacteriaceae possessing different bla genes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediated bla(AmpC), bla(KPC), and bla(NDM) genes using bla gene sequencing as the reference method.
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