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Updated: May 31, 2026
09:07
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
Published on: June 10, 2018
Origins of pluripotent stem cells
B A J Roelen1, S M Chuva De Sousa Lopes
1Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yaleaan, Ultrecht, The netherlands. b.a.j.roelen@uu.nl
Minerva Ginecologica
|July 13, 2011
Summary
This review covers diverse pluripotent stem cell types, including embryonal carcinoma, embryonic stem cells, epiblast stem cells, and induced pluripotent stem cells. It highlights their unique culture conditions and potential differences, especially in epigenetics.
Area of Science:
- Stem Cell Biology
- Developmental Biology
- Cellular Reprogramming
Background:
- Embryonal carcinoma (EC) cells pioneered in vitro pluripotent cell culture.
- Early methods for mouse embryonic stem (ES) cells adapted EC cell culture techniques.
- Human ES cells and mouse epiblast stem cells (EpiSCs) share similar culture requirements.
Purpose of the Study:
- To provide an overview of various pluripotent stem cell types.
- To discuss the culture conditions for different pluripotent stem cells.
- To highlight recent advancements in stem cell derivation and characterization.
Main Methods:
- Review of existing literature on pluripotent stem cell culture.
- Comparative analysis of culture conditions for different stem cell types.
- Discussion of reprogramming techniques for induced pluripotent stem (iPS) cells.
Main Results:
- Identified distinct culture requirements for mouse ES cells, human ES cells, and mouse EpiSCs.
- Demonstrated derivation of pluripotent stem cells from germ cells and epiblasts.
- Introduced iPS cells as a method for patient-specific pluripotent stem cell generation.
- Noted potential epigenetic differences between iPS cells and ES cells.
Conclusions:
- Pluripotent stem cell research has evolved significantly from EC cells to iPS cells.
- Understanding specific culture needs is crucial for each stem cell type.
- Human ES cells may share more similarities with mouse EpiSCs than mouse ES cells.
- iPS cells offer a promising avenue for personalized medicine but require further epigenetic investigation.
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