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Updated: May 31, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
Published on: November 13, 2017
Determinants and dynamics of genome accessibility.
Oliver Bell1, Vijay K Tiwari, Nicolas H Thomä
1Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA. olibell@stanford.edu
Chromatin structure regulates DNA access for essential processes like transcription, replication, and repair. Dynamic control involves DNA sequence, chromatin remodelling, and nucleosome modifications for precise genome regulation.
Area of Science:
- Molecular Biology
- Genetics
- Epigenetics
Background:
- All DNA-templated reactions in eukaryotes occur within chromatin.
- Nucleosome packaging restricts DNA accessibility but also allows regulation via positioning and structure.
- Understanding chromatin's role is crucial for deciphering gene regulation.
Purpose of the Study:
- To review the interplay of factors controlling genome accessibility.
- To discuss how chromatin access regulates transcription, replication, and repair.
Main Methods:
- Review of recent advances in genome-scale methods.
- Analysis of profiles for nucleosome distribution, modifications, and modifiers.
- Integration of DNA sequence, ATP-dependent chromatin remodelling, and nucleosome modifications.
Main Results:
- Genome accessibility is dynamically controlled by multiple factors.
- Detailed profiles reveal complex interplay between DNA sequence and chromatin.
- Chromatin remodelling and nucleosome modifications are key regulatory mechanisms.
Conclusions:
- Dynamic control of genome accessibility is essential for eukaryotic DNA processes.
- The interplay of DNA sequence, chromatin remodelling, and modifications governs gene regulation.
- Chromatin access is a central mechanism for regulating transcription, replication, and repair.
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