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Related Experiment Video

Updated: May 31, 2026

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

Trabecular meshwork gene expression after selective laser trabeculoplasty.

Alberto Izzotti1, Mariagrazia Longobardi, Cristina Cartiglia

  • 1Department of Health Sciences, Faculty of Medicine, University of Genoa, Genoa, Italy.

Plos One
|July 13, 2011
PubMed
Summary

Selective laser trabeculoplasty (SLT) modulates gene expression in the trabecular meshwork, impacting cell functions without causing damage. This research clarifies SLT

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Cell Biology

Background:

  • Trabecular meshwork and Schlemm's canal regulate aqueous humor outflow.
  • Dysfunction leads to increased intraocular pressure.
  • Selective laser trabeculoplasty (SLT) lowers intraocular pressure, but its mechanism is unclear.

Purpose of the Study:

  • Investigate gene expression changes in trabecular meshwork cells post-SLT.
  • Elucidate the molecular mechanisms underlying SLT efficacy.
  • Understand how SLT impacts cellular functions related to aqueous humor outflow.

Main Methods:

  • Primary human trabecular meshwork cells treated with SLT.
  • RNA extracted at 30 minutes, 2 hours, and 6 hours post-treatment.
  • Gene expression analyzed using miRNA-microarray and laser scanner.

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Last Updated: May 31, 2026

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

  • Scanning electron microscopy used for structural analysis.

    • Main Results:

    • SLT modulated genes involved in cell motility, intercellular connections, extracellular matrix, and repair processes.
    • Gene expression changes also noted in reactive oxygen species, antioxidant activity, and inflammation pathways.
    • No phenotypic alterations were observed in trabecular meshwork samples post-SLT.

    Conclusions:

    • SLT influences trabecular meshwork functions at a molecular level.
    • The treatment modulates gene expression without causing cellular or phenotypic damage.
    • SLT's efficacy is linked to its ability to adjust complex tissue functions post-genomically.