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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Aurones: small molecule visible range fluorescent probes suitable for biomacromolecules.

Natasha Shanker¹, Ozlem Dilek, Kamalika Mukherjee

¹Biologics Development Center, Dr. Reddy's Laboratories Ltd, Bachupally, Hyderabad 500072, Andhra Pradesh, India.

Journal of Fluorescence

|July 13, 2011

Summary

New aurone derivatives function as fluorescent probes, with amine substituents enabling visible light excitation. These compounds show potential for biological imaging and photoswitch applications.

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Area of Science:

Natural product chemistry
Photophysics
Biophysical chemistry

Background:

Aurones are natural pigments with reported fluorescence, but limited data exists on their optical properties.
Developing fluorescent probes with visible light excitation is crucial for biological applications.

Purpose of the Study:

Synthesize aurone derivatives as potential fluorescent probes.
Investigate the impact of amine substituents on aurone optical properties.
Explore aurone potential as biological probes and photoswitches.

Main Methods:

Synthesis of four amine-substituted aurone derivatives.
Spectroscopic analysis of absorption and emission properties.
Investigation of photoisomerization in aminoaurones.
Limited testing on protein, nucleic acid, and fixed cells.

Main Results:

Amine substituents shifted absorption to the visible spectrum.
Emission properties strongly depend on substituent and solvent polarity.
Aurones exhibited increased emission intensity and decreased maximum wavelength in less polar solvents.
One aminoaurone derivative demonstrated reversible photoisomerization with distinct emission properties for isomers.

Conclusions:

Substituted aurones are promising fluorescent probes for hydrophobic biological sites.
Aurones can function as photoswitches due to reversible photoisomerization.
Further development of aurone derivatives holds potential for advanced biological imaging and molecular devices.